White Paper on In-Vitro Bioactivity of ASEATM Related to Toxicity, Glutathione Peroxidase, Superoxide Dismutase Efficacy and Related Transcription Factors

This is a technical paper describing a variety of preliminary results from in vitro experiments, performed at national research institutions, investigating the bioactivity of a certain redox signaling compound, ASEATM, when placed in direct physical contact with living cells. Specific investigations include in vitro toxicity and antioxidant efficiencies of the master antioxidants glutathione peroxidase (GPx) and Superoxide Dismutase (SOD) inside living cells and the translocation of two well-studied transcription factors (NFkB, NRF2) known to regulate toxic response and antioxidant production in human cells. Some preliminary work on concentration dependence was also done as well as cell proliferation, counts associated with induced oxidative stress in human cells.